Using the organization to adherentculture, we succeeded in constructing Beijing fatty chicken fibroblast specific target cell.
采用组织块贴壁培养法,成功地构建北京鸡成纤维靶细胞系。
2
Methods Gradient centrifugation and adherentculture were used to get a great amount of NSC and then differentiation antigens were identified.
方法用梯度离心和贴壁培养法分离msc,进行表面分化抗原的鉴定。
3
Artery and vein were removed and mesenchymal tissue was taken out. The primary cells were performed culture and purification with collagenase digestion method to obtain the adherent cells.