The adherent cells were preserved to passage, the purity of ADSCs was analyzed by immunocytochemistry method, and cell growth was observed, then proliferation capability and cell cycle were detected.
Using the organization to adherent culture, we succeeded in constructing Beijing fatty chicken fibroblast specific target cell.
采用组织块贴壁培养法，成功地构建北京鸡成纤维靶细胞系。
3
Conclusion The improved method of whole bone marrow adherent. can successfully isolate and culture BMSCs in rats, which is proved to be an optimal methods for cell preparation and culture.