Suspension single cells were obtained after successive subculturing of 3 or 4 generations.
经3～ 4次继代培养即可得到悬浮的单细胞。
2
Sectoring became visible after subculturing for 5-6 generations, more frequently with 13s8.
菌株角变现象在继代培养5 - 6代后表现明显，而13s8角变子出现的频更高。
3
The shapes of human embryonic lung fibroblasts which were isolated by enzyme digestion were differ, and the culture was all composed of fibroblasts after subculturing for 4-5 generations.