The experiment results showed that first immune should be used to inactivated tissue vaccine in 40d of chinchilla, and it was second immunized when they were 60 days old.

Conclusion Specific expression of MAGE-4 gene was proved in human colonic adenocarcinoma tissue, which indicated that MAGE-4 gene might be used for the development of colonic carcinoma vaccine.

So, we have resorted to developing a waterfowl parvovirus bivalent tissue culture vaccine and have evaluated the potency of the vaccine for reducing the cost of vaccine production.