Mitochondrial targetingsequence may have some problem, it failed to lead the ORF77, ORF17 into mitochondrial, so the protein ORF77, ORF17 didn't work;
线粒体靶向序列可能存在问题,未能将ORF77、ORF17蛋白导入线粒体,所以不能发挥功能;
2
TK contained ALBTRS promoter sequence which probably control the targeting expression in hepatocyte of target gene TK. The enzyme cut assay of the vector LN. ALBTRS.