Objective To explore the feasibility of tissue engineered articular cartilage constructed by the technique of centrifuge tube culture and study its structure and function.
目的探索应用离心管培养技术进行体外构建组织工程化关节软骨组织的可行性，并研究其结构和功能。
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METHODS: Cell culturetechnique and tissue engineering method were used.
方法：采用细胞培养技术和组织工程学方法。
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In this paper the detailed procedures for slice culturetechnique of brain tissue were introduced, including the equipments required, reagents, preparation of brain tissue slice and incubation.