Objective:To analyze the N-terminalaminoacid sequence of bovine cementum attachment protein(CAP), which lay a theoretical foundation for cloning and expression of the CAP.
目的:对牛牙骨质附着蛋白进行N末端氨基酸序列分析,为牙骨质附着蛋白的克隆表达提供一定的理论依据。
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Methods:Various analytical techniques including gene sequencing, aminoacid composition, analysis of N terminalaminoacid analysis, peptide mapping, IEF, molecular weight and UV scanning were used.
The groups divided as above coincided with groups classified according to molecular weight, aminoacid composition and aminoacid sequence of N-terminal of EFE components.