The sterilization method of separating plant endophyte includes the following steps: 1. washing the host sample with endophyte to be separated with bacteria-free water in a bacteria-free work bench;

Survival active substances and bioactive substances can be obtained by extracting and separating fresh plant materials under normal temperature.

Under natural conditions, recent studies had focused on separating the main factors which could effect on intake rates from the set of plant availability variables by experiments.