After a second incubation and washing to remove all the unbound enzyme, a substrate solution is added, which is acted upon by the boundenzyme to produce color.
一会儿之后孵化，洗去移除所有绑定的基体的解酶，补充说，这是通过把作用于酶发生颜色。
2
The starch branching enzymebound to starch granule in rice endosperm was investigated in the present paper.
对水稻胚乳淀粉颗粒结合的淀粉分支酶进行了研究。
3
Some irreversible inhibitors even become covalently bound to amino acids in the active site of the enzyme as they are brought through the early chemical steps of catalysis.