Therefore, the rapid, sensitive and stable semi-nestedPCR are the effective methods for detecting trace bovine and sheep derived materials in feedstuff.
该技术具有快速、灵敏和结果稳定的特点,是检测饲料中痕量牛羊源成分的一种有效方法。
2
Compared with the conventional PCR, semi-nestedPCR appeared to be more sensitive and more practicable, thus providing a reliable method to detect M. suis.
Although using nested or semi-nestedPCR can prominently increase PCR specificity, it really cannot efficiently amplify quick evolving region of DNA in our research of gene Fak56D.