TDZ was advantageous to induction of cotton callus, but it was not advantageous to proliferation and maintain of callus, and was not advantageous to induction of somatic embryoids.
Embryogenic callus and somatic embryos could be directly obtained on the medium only with ZT, but it was influenced by explant sources.
二是先诱导获得愈伤组织，再经继代培养获得胚胎发生。
3
The somatic embryo originated from single embryonic original cell of embryonic callus tissues, which was surrounded by tannin cells, that belonged to single cell origination.