AIM: To study the expression of predicted B cell epitope peptide in S2 subunit of SARScoronavirus spike protein in E. coli and its mimic antigenicity to S2 protein.
Conclusions S2-protein from SARScoronavirus inhibits the chloride channel function in A549 cells, and both PKC and P38 are not involved in mediating this modulation.
To construct a clone p-SARS-S containing SARScoronavirus s gene fragment as the positive control of RT-PCR detect methods, which can build the basis for the expression of the gene.
构建SARS冠状病毒S基因序列克隆p - SARS - S，作为自行设计、建立的RT - p CR检测方法的阳性对照，并为该基因的表达奠定基础。