A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, saltingout, affinity chromatography, ultrafiltration and anion exchange chromatography.
用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法,从牛胎盘中分离纯化出一种肝细胞生长因子。
2
SEC2 was separated and purified successively by ultrafiltration, saltingout with ammonium sulfate, ion - exchange chromatography and molecular exclusion chromatography.