Methods:Culture CHO cells in serum-freemedia and then compare the maintainance time, morphological changes and hemagglutination titers of the cells in culture supernatant.
方法：应用无血清培养基采用小方瓶培养CHO细胞，观察细胞维持时间、培养过程的形态变化。
2
Objective To evaluate the growth of CHO cells in serum-free and serum-containing media.
目的对无血清培养基与含血清培养基培养cho细胞进行比较。
3
PK15 cells were cultured with free-blood serummedia, DMEM media and MEM media in other to observe the effect of cell proliferation, cell transfer and define the ability of nutrition.