Methods:1. Hippocampal neurons of neonatal SD rats were isolated and cultured in the serum-freeculturemedia.
方法：1。采用无血清培养法培养大鼠海马神经元，经免疫荧光法进行纯度鉴定。
2
Methods:Culture CHO cells in serum-freemedia and then compare the maintainance time, morphological changes and hemagglutination titers of the cells in culture supernatant.
方法：应用无血清培养基采用小方瓶培养CHO细胞，观察细胞维持时间、培养过程的形态变化。
3
Instect cell culturemedia were optimized carefully to find new medium for special cell strain and serumfree media was manufactured commercially for suspension cell mass production;