METHODS: the influence of he ne laser irrigation on the growth and development of neurons in hippocampus and cortex of frontal lobe neurons was observed int he cellculture of serumfreemedium.
Instect cellculture media were optimized carefully to find new medium for special cell strain and serumfree media was manufactured commercially for suspension cell mass production;
Conclusions With the application of the "m" serum - freemedium, the technique of Schwann cellculture was simplified and the contamination of fibroblasts was effectively controlled.