METHODS: the influence of he ne laser irrigation on the growth and development of neurons in hippocampus and cortex of frontal lobe neurons was observed int he cellculture of serumfreemedium.
Conclusions With the application of the "m" serum - freemedium, the technique of Schwann cellculture was simplified and the contamination of fibroblasts was effectively controlled.
结论应用“M”无血清培养液简化了雪旺细胞的培养技术，有效地控制了成纤维细胞的污染。
3
Instect cellculture media were optimized carefully to find new medium for special cell strain and serumfree media was manufactured commercially for suspension cell mass production;