Methods Enzyme digestion was performed to isolate single HERG-HEK cell and whole-cell patch-clamp technique was used to record HERG current and kinetic curves.

The types and operating principle of the hydraulic clamp are briefly described, and the structural and parametric designs of the single jaw, normally closed compound hydraulic clamp are detailed.

Methods Cell-attached mode of patch clamp technique were employed to record the single channel current of NMDA receptor with the primary-cultured hippocampal neurons from rats as samples.