The artificial damage of the skeletal muscle during the preparation of singlecellsuspension could cause the earlier degradation of the DNA.
骨骼肌在制成单细胞悬液过程中的人为损伤,可造成DNA提前降解。
2
Place the spleen in a Petri plate with 20 ml of RPMI 10% FBS, cut in two pieces and squeeze each of them between two sterile glass slides to obtain a singlecellsuspension.
Conditioned medium (CM) stemming from cellsuspension culture could remarkably promote the formation of singlecell clone from culture cells of Panax ginseng below a critical cell plating density.