Methods The peptide was separated by ion exchange column chromatography and further purified by reversephaseHPLC, and the molecular weight was determined by tricine gel electro phoresis.
用离子交换柱层析法分离,用反相高效液相色谱法进一步纯化,经聚丙烯酰胺凝胶电泳分析确定相对分子质量。
2
AIM to establish a reversephase high-performance liquid chromatography (RP-HPLC) method to determine the level of tolbutamide in human plasma.
目的:建立反相高效液相色谱法测定人血浆中甲磺丁脲含量的方法。
3
Recombinant human BDNF was expressed in E. coli and is supplied in a lyophilized form. A greater than 96% purity was determined by reversephase-HPLC and SDS-PAGE.