One virus strain isolated by embryo inoculation and serialPassages with suspensions of tung and kidney collected from infected chicks.
通过鸡胚接种、传代,从病鸡肺、肾材料中分离出一株病毒。
2
According to reports, ESC culture in vitro was more than two years and attained 450 serialpassages. Embryonic germline ESC attained 70~80 passages at most.
Infection rates, titer, and OB yield declined with increasing serialpassages of MsNPV in the cell line, they dropped off rapidly after the 7th or 8th passage.