To elucidate the effect of the semi-dry electrophoretic transfer and its influencing factors in Western blot, the protein marker and purified recombination protein were used to analyzed.

This research will facilitate to find new DNA-binding protein, and it is important to reveal the molecular mechanisms of DNA replication and transcription and DNA repair and recombination.

DNA replication, repairing and recombination must depend on the interaction with the protein.