Recombinantclone can be analyzed directly with the use of PCR. It is very efficient, especially in establishment of subtractive library of cDNA.
筛选重组阳性克隆可直接用细菌悬液作PCR模板，在消减文库构建时，能大大提高工作效率。
2
Results and Conclusion: the PCR screening method was convenient and fast for confirming positive recombinantclone, and there was no need for preparation and purification of the plasmid.
结果和结论：以pcr方法筛查重组阳性克隆，可以简便快速鉴定重组阳性克隆，不需提取质粒。
3
Clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.