The results showed that a certain pulse strength was necessary to achieve embryo fusion, and the duration was a key factor affecting the embryo fusion and its later development.

The pulse is differentiated in terms of depth (superficial or deep), speed ( rapid or slow), strength (forceful or weak), shape ( thick or thready, soft or hard) and rhythm.

The effect of pulse parameters on micro-hardness and stretch strength correspond to similar law, but different slightly.