Can you suggest some guidelines to help me validate that the reequilibration is sufficient?
你能告诉我一些规则来帮助我验证这个重新平衡是充分的吗？
2
The reequilibration depends upon the volume of solvent passing through the column at the initial conditions, which is only 8 mL in your case.
这个重新平衡由以初始的比例通过柱子的溶剂体积来决定，对于你的方法它是8分钟。
3
I know the general rule that approximately 10 column volumes of mobile phase is necessary for reequilibration, but I must demonstrate and validate that the reequilibration conditions are sufficient.