The three strategies to construct HAC have been explored without full knowledge of chromosome elements such as centromere, telomere and replicationorigin.
The vector is in ring shape , comprising a procaryon replicationorigin, two eucaryon replication origins and selective marker genes, and two green fluorescent protein gene expression cassettes;
该载 体是一种环状载体，包含原核复制起点、真核复制起点、筛选标记基因和绿色荧光蛋 白基因表达盒；
3
In vitro DNA replication system derived from eukaryotes is capable of replicating exogenous plasmid DNA containing SV40 origin of replication efficiently and accurately.