In order to use retroviral-mediated gene transfer technology in clinical application, retroviral vector must be of high titer and free of detectable replication-competent retroviruses.
逆转录病毒介导的基因转移技术要过渡到临床应用，主要解决如何使病毒载体具有高滴度而不具有复制活性。
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Objective: To construct a replication-competent adenovirus vector carrying human endostatin gene, looking for an effective method for the therapy of colon cancer.