The reactions are typically carried out on a regulated heating block, or PCR machine, and consist of 30-35 cycles of repeated amplification of all the DNA present.
这一反应通常是在一可调控的温箱中或PCR仪中进行，对所有的DNA进行30到35个循环扩增。
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Although this technique has proven its efficiency on several types of cells, its current utilization is limited to the detection of repeated DNA sequences and requires the use of sequential reactions.