A method for gathering and purifying the oil smoke which was produced by oil quenching tanks and tempering in the whole-sealed and constant-temperature heat treatment plant was introduced.
The binding constant, the number of binding sites nand thermodynamic parameters were measured at different temperatures by fluorescence quenching method.
测定了不同温度下该结合反应的结合常数，结合位点数，热力学参数。
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The fluorescent quenching data were analyzed according to Stern -volmer equation and the binding constant was obtained.