Its RNA polymerase activity was dependent upon the primer in vitro with limited reverse transcription activity.
其RNA依赖的RNA聚合酶活性有一定的引物依赖性。
2
This review summarized the recently research process of Sq RT-PCR. It including extraction of total RNA, primer design, definition of cycle count, selection of inner reference and so on.
Methods RNA of virus was obtained from samples of cerebrospinal fluid and serum and then gained with specific primer of Enterovirus through RT-PCR so as to detect specific gene fragment.