Methods LOH at APC and MCC genetic loci in 46 specimens resected from esophageal neoplasm was studied with polymerase chain reaction (PCR) and restrictionfragmentlengthpolymorphism (RFLP).
Methods HBV genotypes were detected by polymerase chain reaction (PCR) and restrictionfragmentlengthpolymorphism analysis (RFLP) in 136 HBV DNA positive patients who were born in Shanghai.
Genome DNA was extracted from white blood cell. Polymerase chain reaction (PCR) and restrictionfragment-lengthpolymorphism (RFLP) were employed to study C-344T polymorphism of CYP11B2 gene.