Result: Compared with normal PCR, Gradient PCR could identify the the optimal annealing temperature of aldosteronesynthase gene.
结果：与普通pcr相比，梯度P CR能够迅速地确定CYP11 B2基因的最佳退火温度。
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Aldosterone secretion is regulated largely at the level of expression of the final enzyme required for its biosynthesis, aldosteronesynthase (CYP11B2).
Several genetic polymorphisms of the aldosteronesynthase gene (CYP11B2), which may influence the plasma aldosterone levels, had been reported to influence the blood glucose levels.