A specific semi-nested RT-PCR based on the RNA-dependent RNA polymerase gene in AF469603 of Chinese bee (Apis sinensis) sacbrood virus was established to detect Chinese bee sacbrood virus.

Eligible patients were hepatitis B surface antigen-positive men and women with compensated liver disease who were given lamivudine at least more than 6 months and had HBV polymerase gene mutation.

AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.