The assay begins with a standard kinase reaction performed in the provided reactionBuffer with a provided Bisamide Rhodamine 110 peptide substrate (PKA R110 substrate).
检测开始时、先用试剂盒提供的PKA双酰胺罗丹明110肽底物进行一个标准的激酶反应。
2
The acidity for hydride generation reaction, selection of buffer solution and oxidizer, dynamic linear range, interference from coexistent elements and their elimination were studied.