The yield of callus increased apparently by higher temperature preculture for a short time.
短期高温预培养可明显提高愈伤组织的产量;
2
We evaluated the effects of the duration of sonication treatment, length of explant preculture, addition of acetosyringone (AS) and the orientation of the explants during the co cultivation...
并对超声波处理时间、外植体放置方向、预培养时间、外植体类型、乙酰丁香酮等影响因素进行了优化。
3
Wheat genotypes Yangmai 158, Yangmai 10 are excellent transformation materials. Wheat immature embryos with 4 days preculture have high tissue culture response and strong regeneration capacity.