The bovine, sheep and goat derived materials were positive in mixed feed powders of level test by PCR and restrictionenzymeanalysis.
P CR检测和酶切鉴定结果表明,在水平测试的混合饲料粉中可检出牛源、羊源性成分。
2
Results Restrictionenzymeanalysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.
结果酶切和测序证实PTEN基因克隆和真核表达载体构建成功。
3
Plasmid DNAs were prepared by alkali lysis and purified with polyethylene glycol 8000. Restrictionenzymeanalysis show that both genes are well-constructed suitable for transgenic animal experiment.