Results and Conclusion: the PCR screening method was convenient and fast for confirming positive recombinant clone, and there was no need for preparation and purification of the plasmid.
结果和结论：以pcr方法筛查重组阳性克隆，可以简便快速鉴定重组阳性克隆，不需提取质粒。
2
The effects of concentration of NaOH and SDS temperature and duration of incubation on the isolation and purification of large plasmid DNA of Shigella were studied.