To construct a recombinant plasmid containing protein transduction domain (PTD) and brain derived neurotrophic factor (BDNF) fusion gene and express in e.
构建含蛋白转导结构域(PTD)与脑源性神经营养因子(BDNF)融合基因的质粒,并在大肠肝菌中表达。
2
Objective To construct recombinant plasmid with human atrial natriuretic peptide (ANP) gene in fusion form for stable and high level expression of genetic engineering product ANP in E. coli system.
目的构建以融合蛋白形式在大肠杆菌中高效表达心钠素的重组质粒,稳定高效地获得基因工程产品心钠素。
3
RESULTS: DDR2/EGFP fusionplasmid was successfully constructed. Further analysis also demonstrated that the fusion protein has similar expression and activation pattern with wild type ones.