Objective To establish an efficient method for isolation and culture of mesenchymal stem cells from mouse bonemarrow.
目的建立一种从小鼠骨髓中分离培养间充质干细胞的高效方法。
2
Methods Chromosome specimens were prepared by short term culture of bonemarrow cells and karyotype analyses were carried out using R and G banding techniques.
方法应用骨髓细胞短期培养法制备染色体标本，并应用R和G显带技术进行核型分析；
3
Methods Sterility test, mycoplasma detection, pyrogen detection and abnormal toxicity test were performed respectively on culture supernatant of neural stem cells from bonemarrow.