Objective To examine the changes of conformation and activity of human placentalalkalinephosphatase(HPAP) in denaturation and renaturation of guanidine chloride, and to study its folding mechanism.
目的观测人胎盘碱性磷酸酶（HPAP）在盐酸胍变性和复性过程中构象与活力的变化，探讨其折叠机制。
2
Objective To study the effects of phosphate on guanidine denaturation of human placentalalkalinephosphatase (HPAP) and provide a theoretical basis for flexibility hypothesis of enzyme active sites.