It begins with the bond of the factors assembling at the start of the gene, that is a linear sequence in DNA instructions.
这个过程是从复合在基因开头的绑定因子上开始，该因子是一个线性的DNA调控序列。
2
The bond is broken releasing the 5 'end of the intron and attaching it to the branch point sequence, the intron now forms a tailed loop structure called a lariat.