Methods Rat gastric parietal cells were isolated by pronase digestion and purified by discontinuous density-gradient centrifugation.
方法采用链霉蛋白酶消化、间断密度梯度离心纯化大鼠胃黏膜壁细胞。
2
MethodsAfter rat hippocampal or cerebral cortical neurons were isolated by pronase e, the IA currents were recorded by whole-cell patch clamp technique.
方法用急性酶解法获得单个大鼠心室肌细胞，标准的全细胞膜片钳技术记录钠通道电流。
3
The sperm-oocyte binding was markedly inhibited by the glycopeptide from BSL degradated by pronase E, suggesting that the glycopeptide of BSL may also take part in sperm-oocyte binding.