An oblique hole through the glass pipetteholder above the lateral hole for cell seal suction is drilled, through which a microcatheter o.
利用通用的微电极夹持器在其抽吸负压的侧管上方钻一斜孔直通夹持器中央管腔。
2
In patch clamp experiment, the formation of giga-ohm seal is a critical step, which is usually fulfilled through controlling the air pressure within pipette and the holder.