Methods: In vitro cultured rat skin flbroblast cells, MTT assay and agaroverlay were used to evaluate toxicity grading of GMO on skin flbroblast cells.
Red cell 6 Phosphogluconate dehydrogenase (6 PGD) and glucose phosphate isomerase (GPI) in Shandan horses were studied by use of starch gels with using AGARoverlay technique.
The relationship between virulence and the size of the plaques of recombinant vaccinia viruses was studied by plaque assay method on chicken embryo cell monolayer with AGARoverlay.