Red cell 6 Phosphogluconate dehydrogenase (6 PGD) and glucose phosphate isomerase (GPI) in Shandan horses were studied by use of starch gels with using AGAR overlay technique.
Sclerotia could germinate in 0.05% glucose solution, distilled water and water agar, and the germination percentage is the highest in 0.05% glucose solution.
菌核在0.05%葡萄糖溶液、蒸馏水、水琼脂上均可萌发，以0.05%葡萄糖溶液萌发最好。
3
Just before use, heat the glucoseAGAR tube in boiling water or flowing steam for 15 min, then cool rapidly to the incubation temperature.