Conclusions RCC cells can be amplified by passageCulture, but only within the cells of four generations.
结论软骨细胞可以通过传代培养获得扩增,但仅限于四代以内细胞。
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This review focuses on the methods of isolation and cultivation of pathogenic free-living amoebae, including sample treatment, culture conditions, passageculture, pathogen detection, and maintenance.