A new electrochemical voltammetric enzyme immunoassay system was investigated based on p-nitrophenyl phosphate (PNPP)-alkaline phosphatase (ALP) coupling reaction.
研究了对硝基磷酸苯酯(PNPP)为底物伏安法测定碱性磷酸酯酶(ALP)的方法。
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Serum ALP in LDG and MDG were higher than normal group(P<0.01 and P<0.01) but showed no difference between HDG and normal group(P >0.05).
The PD and GCF-ALP of control group detected in 6 and 12 months after operation had no significant differences compared with those detected before operation(P>0.05).