Additionally, three herbicides could induce a variety of malformations on X. tropicalis embryos, such as abdominal swelling, facial deformities, curvature of the spine, tail bent and head deformities.

Xylene was used to induce mouse ear swelling, formaldehyde was used to induce mouse foot swelling and ice-acetic acid was used to increase blood capillary leakage in mouse abdominal cavity.