By particlebombardment method, 167 regenerated plantlets were gained from 1160 calli, and the transformation frequency was 17.4%.
通过基因枪转化法轰击1160块的愈伤组织得到167株再生植株,转化率17.4%。
3
The direct gene transfer systems involved the chemical method (PEG), electroporation, microinjection, particlebombardment, pollen-mediated gene transfer and other systems using various vectors.