Two dimensional polyacrylamide gel electrophoresis(2DPAGE) followed by mass spectrometry(MS) is the most widely used method of protein resolution and identification. But it exist shortcomings.
双相聚苯稀酰氨凝胶电泳（2 D PAGE）与质谱（MS）是分辨和鉴定蛋白的最常用的方法，但也存在有缺点。
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Methods nuclear matrix proteins were extracted under high salt extraction procedures, and changes of nuclear matrix proteins were analysed by SDS PAGE and two dimensional (2d) electrophoresis.
So in this paper we adopt 2D-PAGE to analysis the different serum protein group of healthy rat , cardiac muscle bloodless rat, treated drug-taken rat so that we may discuss the medication effect.